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1.
Braz. arch. biol. technol ; 61(spe): e18000014, 2018. tab, graf
Article in English | LILACS | ID: biblio-974150

ABSTRACT

ABSTRACT The global use of fossil energy sources has led to the need to develop technologies capable of mitigating its consequences. One such option is anaerobic biodigestion, which is already scientifically well-established, although the use of its main products (biogas and biomethane) still requires further development before they can be employed in automotive vehicles, largely due to technical issues concerning their storage. The storage of methane using adsorption (Adsorbed Natural Gas - ANG) is a technology that requires less energy and moderate pressures and temperatures. However, the main storage techniques continue to be those that involve high pressures and/or cryogenic temperatures. The present work evaluates the performance of an unprecedented prototype developed by UFPR for the storage of methane by adsorption, employing commercial activated carbon. The factorial design (2²) indicated that the storage capacity of the system was 39.41 V /V using activated carbon, and the desorption process was effective at 60ºC. Thus, the use of adsorbents of low cost and easy access can provide very interesting results (>100 V / V), which contributes to make the technology viable.


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Charcoal/administration & dosage , Adsorption , Biofuels
2.
Braz. arch. biol. technol ; 48(spe): 85-95, June 2005.
Article in English | LILACS-Express | LILACS, SES-SP | ID: lil-415461

ABSTRACT

Desenvolvimento de meios de cultura isentos de soro fetal bovino (SFB) é uma das grandes prioridades de pesquisa em desenvolvimento de processos com célula animal. O objetivo do presente trabalho foi realizar uma análise do potencial de uso da hemolinfa como suplemento do meio utilizado no cultivo da célula animal ancorante CHO-K1. Para isso, foi adicionado 1% v/v de extrato de hemolinfa ao meio DMEM contendo 10% v/v de SFB e 1,0 ou 4,5 g/L de glicose. O cultivo foi realizado em frascos tipo spinner em um ambiente de 10% v/v de CO2, a 37ºC, utilizando o microcarregador Cytodex 1. Comparando os resultados obtidos no ensaio com hemolinfa com um sem hemolinfa pode-se notar uma influência positiva da hemolinfa no cultivo, já que o ensaio com hemolinfa apresentou uma concentração máxima de células 52% maior e uma produtividade máxima de até 40% maior.

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